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Immunology and Infection
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Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

DOI:
10.3791/50667-v

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07:07 min

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September 12, 2013

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, , ,
1Department of Molecular and Biomedical Sciences,University of Maine

Chapters

  • 00:05Title
  • 01:02Zebrafish Care and Maintenance
  • 01:59Microplate Reader Programming
  • 03:57Fluorescence Quantification
  • 04:34Data Analysis
  • 05:41Results: Respiratory Burst Response in Zebrafish Embryos
  • 06:41Conclusion

Summary

Automatic Translation

Automatic Translation

The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.

Tags

Keywords: Respiratory BurstInnate ImmunityZebrafishReactive Oxygen SpeciesPhagocyteFluorometric AssayMicroplate ReaderFluorogenic SubstrateDevelopmental StageKidneyFish Species

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