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Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
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Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Article DOI: 10.3791/51289-v • 10:30 min • May 23rd, 2014
May 23rd, 2014 •
1, 2, 2, 1
1Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute, 2Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School

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Summary
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Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.

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In Vivo Imaging Osteogenic Stem Cells Progenitor Cells Fracture Repair Bone Turnover Regenerative In Vivo Demonstration Osteo-lineage Cell Tracing Models Microfractures Calvarial Bone Injury Sites Osteoblasts Stem Cell-intrinsic Regulators Extrinsic Molecular Regulators Bone Regeneration Bone Repair
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