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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
JoVE Journal
Biology
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JoVE Journal Biology
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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14:51 min

November 13, 2021

DOI:

14:51 min
November 13, 2021

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Summary

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The described protocol provides an optimized quantitative proteomics analysis of tissue samples using two approaches: label-based and label free quantitation. Label-based approaches have the advantage of more accurate quantitation of proteins, while a label-free approach is more cost-effective and used to analyze hundreds of samples of a cohort.

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