61,146 Views
•
13:26 min
•
September 13, 2014
DOI:
Summary
Automatically generated
Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.
Related Videos
10:29
Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
Related Videos
113184 Views
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Related Videos
29839 Views
11:00
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
Related Videos
12105 Views
10:17
Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
Related Videos
12148 Views
13:51
The MultiBac Protein Complex Production Platform at the EMBL
Related Videos
15811 Views
08:29
Using Coculture to Detect Chemically Mediated Interspecies Interactions
Related Videos
13471 Views
09:35
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Related Videos
22492 Views
11:32
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Related Videos
13158 Views
03:28
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Related Videos
19361 Views
06:55
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
Related Videos
2804 Views
Read Article
Cite this Article
Vivoli, M., Novak, H. R., Littlechild, J. A., Harmer, N. J. Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry. J. Vis. Exp. (91), e51809, doi:10.3791/51809 (2014).
Copy