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JoVE Journal
Biology

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Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
 

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Article DOI: 10.3791/51809
September 13th, 2014

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Summary September 13th, 2014

Differential scanning fluorimetry is a widely used method for screening libraries of small molecules for interactions with proteins. Here, we present a straightforward method to extend these analyses to provide an estimate of the dissociation constant between a small molecule and its protein partner.

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