Journal
/
Neuroscience
/
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

DOI:
10.3791/61110-v

•

06:47 min

•

May 21, 2020

•

, , , , , , , , , ,
1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris

Chapters

  • 00:04Introduction
  • 00:43Surgical Preparation
  • 01:39Pregnant Mouse Preparation
  • 02:16Intraventricular Injection
  • 03:16In Utero Electroporation (IUE)
  • 04:24Multichannel Confocal Imaging
  • 05:23Results: Representative Cortical Astrocyte Segmentation and Arborization
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.

Tags

Keywords: In Utero ElectroporationMAGIC MarkersCortical AstrocytesIndividualizationMorphology AssessmentProgenitor Cell LabelingNeuronal And Viral ProgenyMicropipetteDNA SolutionLateral Ventricle InjectionEmbryonic Day 15.5Surgical Procedure

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61110?article_type=v

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
check_url/61110?article_type=v

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
check_url/61110?article_type=v

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
check_url/61110?article_type=v

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
check_url/61110?article_type=v

Isolation and Culture of Mouse Cortical Astrocytes
check_url/61110?article_type=v

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
check_url/61110?article_type=v

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
check_url/61110?article_type=v

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
check_url/61110?article_type=v

Subtype-selective Electroporation of Cortical Interneurons
check_url/61110?article_type=v

Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse

Read Article