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JoVE Journal Neuroscience
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In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

Article DOI: 10.3791/61110-v • 06:47 min • May 21st, 2020
May 21st, 2020 •
*1, *2, 1, 3, 4, 2, 1, 4, 3, 2, 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
* These authors contributed equally

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Summary
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Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.

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In Utero Electroporation Multiaddressable Genome-Integrating Color (MAGIC) Markers Individualize Cortical Mouse Astrocytes Technique Label Multiple Progenitor Cells Neuronal Progeny Viral Progeny Precise Control Sterilize Surgical Tools Glass Bead Sterilizer Electrode Gel Micropipette Micro Injection Holder DNA Solution Fast Green Solution Pressure Parameter Time Parameter Micro Injector Pregnant Mouse Anesthetic Injection
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