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Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Immunology and Infection
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JoVE Journal Immunology and Infection
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

English

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10,300 Views

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13:36 min

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May 06, 2015

DOI:

10.3791/52655-v

DOI:
10.3791/52655-v

•

13:36 min
May 06, 2015

•

1 Views
, , ,
1Department of Medicine,University of Alabama at Birmingham, 2Center for AIDS Research,University of Alabama at Birmingham

Summary

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Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.

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Cite this Article

Gu, L., Farrow, A. L., Krendelchtchikov, A., Matthews, Q. L. Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches. J. Vis. Exp. (99), e52655, doi:10.3791/52655 (2015).

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