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Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

DOI:
10.3791/56372-v

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09:03 min

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January 03, 2018

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, ,
1CELLINK LLC, 2CELLINK AB, 33D Bioprinting Centre, Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Chapters

  • 00:05Title
  • 00:51Preparation of Consumables, Bioink, and Cells
  • 02:33Mixing of Cell Suspension and Bioink
  • 04:44Bioprinting of Cartilage Analogs with a Single Cell type
  • 07:28Results: Cell viability after Mixing and During Long-term Culture
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Cartilage and skin analogs were bioprinted using a nanocellulose-alginate based bioink. The bioinks were cellularized prior to printing via a single step passive mixing unit. The constructs were demonstrated to be uniformly cellularized, have high viability, and exhibit favorable markers of differentiation.

Tags

Keywords: BioprintingCartilagePassive Mixing UnitBioink PrecellularizationCell ViabilityMixing RatioNanocellulose AlginateCalcium ChlorideCell SuspensionTrypan BlueCell DensityFibroblastsDispensing UnitLuer Lock

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