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May 10, 2015
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The current protocol details a method for measuring the activity of functionally homologous deubiquitinating enzymes. Specialized probes covalently modify the enzyme and allow for detection. This method holds the potential to identify new therapeutic targets.
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Cite this Article
Griffin, P., Sexton, A., Macneill, L., Iizuka, Y., Lee, M. K., Bazzaro, M. Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples. J. Vis. Exp. (99), e52784, doi:10.3791/52784 (2015).
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