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Neuroscience

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Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
 

Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

Article doi: 10.3791/59695
May 21st, 2019

Summary May 21st, 2019

This protocol is designed to efficiently quantify ubiquitin-proteasome system (UPS) activity in different cellular compartments of the rodent brain. Users are able to examine UPS functioning in nuclear, cytoplasmic and synaptic fractions in the same animal, reducing the amount of time and number of animals needed to perform these complex analyses.

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