Measurement of Cellular Chemotaxis with ECIS/Taxis

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Measurement of Cellular Chemotaxis with ECIS/Taxis

DOI:

10.3791/3840-v

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11:37 min

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April 01, 2012

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Kathryn M. Pietrosimone, Xiuyin Yin, David A. Knecht, Michael A. Lynes

¹Molecular and Cell Biology,University of Connecticut, ²University of Connecticut

Chapters

00:05Title
01:28ECIS/Taxis Electrode Preparation
03:13Preparing Agarose Chambers and the Cannula Well-cutting Tool
04:21Cutting Wells into Agarose
05:51Loading the Wells
07:04Data Collection
08:55Data Management
10:13Results: Chemotaxis of Human Jurkat T cells in Response to SDF1alpha
11:14Conclusion

Summary

Automatic Translation

The ECIS/Taxis system is an automated, real-time assay that measures cellular chemotaxis. In this assay, cells move beneath a layer of agarose to arrive at a target electrode. Cellular movement is measured by the onset of resistance to AC current 0.

Measurement of Cellular Chemotaxis with ECIS/Taxis

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