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A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Article DOI: 10.3791/61783-v • 06:38 min • January 22nd, 2021
January 22nd, 2021 •
1,2, 1,2, 1, 1,2
1Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 - IRFAC INSERM, Université de Strasbourg

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Summary
Automatic Translation

This protocol presents a novel, robust, and reproducible culture system to generate and grow three-dimensional spheroids from Caco2 colon adenocarcinoma cells. The results provide the first proof-of-concept for the appropriateness of this approach to study cancer stem cell biology, including the response to chemotherapy.

Tags

Three-dimensional Model Spheroids Colon Cancer Stem Cells Tumorigenicity Cancer Stem Cells High Throughput Screen New Drugs Homogeneously Sized Spheroids Chronic Cancer Biology 3D Culture Conditions Anti-Adherence Rinsing Solution Basal Medium DMEM Basement Membrane Matrix Medium Caco-2 Cells
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