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A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
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Cancer Research
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JoVE Journal Cancer Research
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

DOI:
10.3791/61783-v

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06:38 min

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January 22, 2021

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, , ,
1Département de la recherche,Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 – IRFAC INSERM, Université de Strasbourg

Chapters

  • 00:04Introduction
  • 01:05Spheroid Culture Preparation
  • 01:47Spheroid Generation
  • 03:24Spheroid Harvest
  • 04:30Results: Representative Colon Cancer Stem Cell Spheroid Formation and Analysis
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a novel, robust, and reproducible culture system to generate and grow three-dimensional spheroids from Caco2 colon adenocarcinoma cells. The results provide the first proof-of-concept for the appropriateness of this approach to study cancer stem cell biology, including the response to chemotherapy.

Tags

3D Spheroid ModelColon Cancer Stem CellsTumorigenicityCancer Stem Cell AnalysisHigh-throughput Drug ScreeningHomogeneous Spheroid Culture3D Culture ConditionsCaco-2 CellsDMEM Basement Membrane Matrix MediumCell CountingCell SeedingCentrifugationMicroscopic Observation

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