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Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics
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Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

DOI:
10.3791/53958-v

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11:28 min

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May 11, 2016

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, , , , ,
1UCBL1, UMR/CNRS 5822, Laboratoire de Radiobiologie Cellulaire Moléculaire,Université de Lyon, 2Hospices-Civils-de-Lyon, Centre Hospitalier Lyon-Sud

Chapters

  • 00:05Title
  • 00:55Side Population (SP) Selection
  • 04:20CD44highALDHhigh Sub-population Selection
  • 08:12Cell Culture and Tumor Potential and CSC Characteristic Confirmation
  • 09:13Results: Confirmation of CD44highALDHhigh Cell Tumor Potential
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

Understanding the role of cancer stem-like cells in tumor recurrence and resistance to therapies has become a topic of great interest in the last decade. This article describes the isolation and characterization of the sub-population of cancer stem-like cells from head and neck squamous carcinoma cell lines (HNSCC).

Tags

Keywords: Head And Neck Squamous Cell CarcinomaCancer Stem CellsHoechst Dye Efflux AssaySide PopulationFlow CytometryVerapamilPropidium IodideCell CultureCell IsolationCell Characterization

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