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Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

DOI:
10.3791/62456-v

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09:45 min

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April 12, 2021

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, , ,
1Department of Pediatrics,University of California, San Diego School of Medicine, 2Sanford Consortium for Regenerative Medicine, 3Sanford Burnham Prebys Medical Discovery Institute

Chapters

  • 00:04Introduction
  • 01:05Definitive Endoderm Induction from Induced Pluripotent Stem Cells (Day 1-3)
  • 03:13Anterior Foregut Endoderm (AFE) Induction and Lung Progenitor Cell (LPC) Differentiation
  • 05:003D lung Organoid Induction (Day 16 22)
  • 06:493D Lung Organoid Branching and Maturation
  • 07:11Results: Characterization of Endoderm and 3D Whole Lung Organoids
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

The article describes step wise directed differentiation of induced pluripotent stem cells to three-dimensional whole lung organoids containing both proximal and distal epithelial lung cells along with mesenchyme.

Tags

Keywords: 3D Whole Lung OrganoidsInduced Pluripotent Stem CellsLung Developmental BiologyLung Disease ModelingCryopreservationGenetic ManipulationDefinitive Endoderm InductionROCK InhibitorGFR Basement Membrane Matrix

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