Journal
/
Biology
/
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines

DOI:
10.3791/54350-v

•

12:35 min

•

September 03, 2016

•

, ,
1Medical Genetics,Hunter Medical Research Institute, 2Priority Research Centre for Cancer, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,University of Newcastle, 3Pathology North,John Hunter Hospital

Chapters

  • 00:05Title
  • 00:38Preparing Cells for Proliferation Assays
  • 02:49Determining Cell Count Using a Hemocytometer
  • 04:21Determining Cell Proliferation Using Luminescence-based Assay
  • 07:02Determining Cell Count Using a Cell Imager
  • 10:40Results: Comparison of Three Different Methods Measuring Cell Proliferation in Breast Cancer Cell Lines
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.

Tags

Keywords: Cell ProliferationBreast Cancer Cell LinesCell CountingCell ImagerMCF-7 CellsTrypsinDMEMDPBSHemocytometerCell SeedingCell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54350?article_type=v

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.
check_url/54350?article_type=v

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
check_url/54350?article_type=v

Determining Cell Number During Cell Culture using the Scepter Cell Counter
check_url/54350?article_type=v

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
check_url/54350?article_type=v

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
check_url/54350?article_type=v

Methods for Skin Wounding and Assays for Wound Responses in C. elegans
check_url/54350?article_type=v

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
check_url/54350?article_type=v

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
check_url/54350?article_type=v

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
check_url/54350?article_type=v

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts

Read Article