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Legionella pneumophila ydermembranvesikler: Isolering og analyse af deres pro-inflammatorisk potentiale på makrofager
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
DOI:

08:34 min

February 22, 2017

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Chapters

  • 00:05Title
  • 01:18Cultivate L. pneumophila and Prepare and Quantify L. pneumophila Outer Membrane Vesicles (OMVs)
  • 03:42Pre-treat, Infect Macrophages, and Assess Bacterial Replication with a Colony-Forming Unit (CFU) Assay
  • 05:31Results: Pro-inflammatory Response of Macrophages from L. pneumophila Infection
  • 07:04Conclusion

Summary

Automatic Translation

Her beskriver vi oprensningen af Legionella pneumophila (L. pneumophila) ydre membranvesikler (OMV'er) fra flydende kulturer. Disse oprensede vesikler anvendes derefter til behandling af makrofager til at analysere deres pro-inflammatoriske potentiale.

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