Journal
/
Immunology and Infection
/
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

DOI:
10.3791/65062-v

•

06:28 min

•

February 17, 2023

•

, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Chapters

  • 00:04Introduction
  • 00:42Cell Culture
  • 01:44Isolation of Extracellular Vesicles
  • 03:14Western Blotting
  • 04:47Results: Characterization of Extracellular Vesicles
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Tags

Keywords: Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/65062?article_type=v

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
check_url/65062?article_type=v

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
check_url/65062?article_type=v

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
check_url/65062?article_type=v

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
check_url/65062?article_type=v

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
check_url/65062?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/65062?article_type=v

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
check_url/65062?article_type=v

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
check_url/65062?article_type=v

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
check_url/65062?article_type=v

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

Read Article