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Immunology and Infection
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Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

DOI:
10.3791/58775-v

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08:38 min

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February 22, 2019

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, , ,
1Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 2Department of Microbiology & Immunology,University of Illinois College of Medicine

Chapters

  • 00:04Title
  • 01:08Infection Procedures
  • 02:16Sampling Processing of Intracellular and Emergent Lm for Colony-forming Unit (CFU) Assay
  • 03:58Sampling Processing of Intracellular and Emergent Lm for Flow Cytometry (FCM)
  • 06:22Results: The Role of Pathogen and Host-encoded Factors on Cellular Infection
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

We describe both in vitro and in vivo infection assays that can be used to analyze the activities of host-encoding factors.

Tags

Bacterial PathogenHost ResponseIntracellular ReplicationPathogen EffluxInfection ExperimentGentamicinCell LysisDilutionAgar Plating

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