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Vurdering av neuronal levedyktighet ved bruk av fluoresceindiacetat-propidiumjodid-dobbeltfarvning i cerebellar granulærneuronkultur
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1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Chapters
- 00:05Title
- 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
- 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
- 05:30Assessment of Neuronal Viability
- 07:08Results: Double and Triple Stainings in CGN Culture
- 08:19Conclusion
Denne protokollen beskriver hvordan man nøyaktig måler neuronal levedyktighet ved å bruke fluoresceindiacetat (FDA) og propidiumjodid (PI) dobbeltfarging i dyrkede cerebellargranulæreuroner, en primær nevronkultur som brukes som en in vitro- modell i nevrovitenskapelig og neurofarmakologisk forskning.
Tags
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeuroscienceNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
