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JoVE Journal
Genetics

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Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
 

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Article DOI: 10.3791/57410
March 20th, 2018

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Summary March 20th, 2018

Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.

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