A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:46Reagents and Media Preparation
- 01:59Aspirator Setup
- 02:37High-Throughput Liquid Surface Sterilization of Seeds
- 04:56Plating and Scoring of Arabidopsis on Half Strength Murashige and Skoog Plates
- 05:32Results: Germination Analysis of Arabidopsis Seeds After Surface Sterilization with the New Method
- 06:57Conclusion
A high-throughput protocol for the surface sterilization of Arabidopsisthaliana (Arabidopsis) seeds is provided, optimizing the liquid handling steps with a simple suction device constructed with a vacuum pump. Hundreds of seed samples can be surface-sterilized in one day.
