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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Article DOI: 10.3791/51265-v • 14:44 min • March 14th, 2014
March 14th, 2014 •
1, 1
1Department of Biology, Technion - Israel Institute of Technology

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Many mRNAs encoding mitochondrial proteins are associated with the mitochondria outer membrane. We describe a subcellular fractionation procedure aimed at isolation of yeast mitochondria with its associated mRNAs and ribosomes. This protocol can be applied to cells grown under diverse conditions in order to reveal mechanisms of mRNA localization and localized translation near the mitochondria.

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Isolation MRNAs Yeast Mitochondria Mechanisms Localized Translation Mitochondrial Proteins Import Synthesized Mitochondria Vicinity Ribosomes Outer Membrane Translation Process Import Efficiency Regulation Sites Ectopic Expression Factors Elements Subcellular Fractionation Differential Centrifugation Protocol Isolation Of MRNAs Ribosomes Proteins Molecular Methods Biochemical Methods Transcriptomics Proteomics Methods Yeast Model Organism Speed Costs Simplicity Genetic Tools Deletion Strains
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