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JoVE Journal Genetics
En Optogenetic metod för att kontrollera och analysera gen uttrycksmönster i Cell-till-cell interaktioner
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En Optogenetic metod för att kontrollera och analysera gen uttrycksmönster i Cell-till-cell interaktioner

Article DOI: 10.3791/57149-v • 07:59 min • March 22nd, 2018
March 22nd, 2018 •
1,2, 1,3,4,5
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

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Här presenterar vi ett protokoll för att analysera cell till cell överföring av oscillerande information av optogenetic kontroll och live övervakning av genuttryck. Detta tillvägagångssätt ger en unik plattform för att testa en funktionell betydelse av dynamiska gen uttryck program i flercelliga system.

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Fråga 133 realtid imaging optogenetic metod genetik luciferas reporter GAVPO oscillerande uttryck Notch signalering
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