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Bioengineering

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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
 

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Article DOI: 10.3791/62109-v
July 6th, 2021

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Summary July 6th, 2021

Reliably controlling light-responsive mammalian cells requires the standardization of optogenetic methods. Toward this goal, this study outlines a pipeline of gene circuit construction, cell engineering, optogenetic equipment operation, and verification assays to standardize the study of light-induced gene expression using a negative-feedback optogenetic gene circuit as a case study.

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