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Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope
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Bioengineering
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JoVE Journal Bioengineering
Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

DOI:
10.3791/57797-v

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07:02 min

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July 03, 2018

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, , , , ,
1Department of Mechanical & Aerospace Engineering,Old Dominion University, 2Department of Biological Sciences,Old Dominion University

Chapters

  • 00:04Title
  • 00:42Soft Silicone Substrate Fabrication
  • 01:40Fluorescent Microbead-Soft Silicone Coupling
  • 03:34Measurement of Silicone Stiffness with Sphere Indentation
  • 04:39Silicone Stiffness Calculation (Young s Modulus)
  • 05:51Results: Representative Microbead Images and Stiffness Values
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Substrates with stiffness in the kilopascal-range are useful to study the response of cells to physiologically relevant micro-environment stiffness. Using just a widefield fluorescence microscope, the Young's modulus of soft silicone gels can be determined using an indentation with a suitable sphere.

Tags

Keywords: Stiffness MeasurementSoft Silicone SubstratesMechanobiologyWidefield Fluorescence MicroscopeYoung’s ModulusPolyacrylamide GelSelf-stretching ExperimentsSoft Silicone ElastomerAir BubblesDeep UV TreatmentEDCSulfo-NHSFluoro-4 Conjugated Carboxylate-modified Micro-beadsRat Tail Collagen 1

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