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Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

DOI:
10.3791/57546-v

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08:56 min

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May 11, 2018

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, ,
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences,National Defense Medical Center, 2Institute of Molecular Biology,Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture,National Taiwan University

Chapters

  • 00:04Title
  • 00:56Fixation and Whole-mount Immunostaining
  • 02:55Sample Set-up
  • 05:32Quantification of Axon Arborization
  • 07:27Results: Visualization and Quantification of Motor Axon Arborization
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol for visualizing motor neuron projection and axon arborization in transgenic Hb9::GFP mouse embryos. After immunostaining for motor neurons, we used light sheet fluorescence microscopy to image embryos for subsequent quantitative analysis. This protocol is applicable to other neuron navigation processes in the central nervous system.

Tags

Keywords: Motor AxonAxon ArborizationLight Sheet Fluorescence MicroscopyMouse EmbryosMotor Neuron DevelopmentGFPAntibody StainingImage Analysis

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