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Journal
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Biochemistry
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全飞头段耗氧率的测定与解释
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Please note that all translations are automatically generated.
Click here for the English version.
全飞头段耗氧率的测定与解释
DOI:
10.3791/58601-v
•
07:11 min
•
January 07, 2019
•
Louisa Jutta Dietz
,
Anuroop Venkateswaran Venkatasubramani
,
Annika Müller-Eigner
,
Martin Hrabe de Angelis
4,5
,
Axel Imhof
,
Lore Becker
,
Shahaf Peleg
6,7
1
Munich Center of Integrated Protein Science and Biomedical Center
,
Ludwig-Maximilians University of Munich
,
2
Laboratory for Metabolism and Epigenetics in Aging
,
Leibniz Institute for Farm Animal Biology (FBN)
,
3
German Mouse Clinic, Helmholtz Zentrum Munich
,
German Research Center for Environment and Health (GmbH)
,
4
German Center for Diabetes Research (DZD)
,
5
Chair of Experimental Genetics, School of Life Science Weihenstephan
,
Technische Universität München
,
6
Laboratory for Metabolism and Epigenetics in Brain Aging
,
Institute of Neuroregeneration & Neurorehabilitation of Qingdao University
,
7
Molecular Biology Division, Biomedical Center, Faculty of Medicine
,
Ludwig-Maximilians University of Munich
Chapters
00:04
Title
00:33
Instrument Preparation
01:24
Cartridge Preparation
02:21
Plate Preparation
04:37
Analysis of OCR Measurements
05:12
Results: Oxygen Levels of Midlife Heads Lower than Young Heads
06:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
测量代谢率的变化是了解各种疾病和衰老进展的关键。在这里, 我们提出了一个新的技术来测量整个头部的耗氧量, 更接近的生理状态, 并可能有助于揭示新的药物, 改变线粒体的活性。
Tags
Oxygen Consumption Rate
Metabolism
Aging
Mitochondrial Isolation
Calibration
Experimental Drugs
Fly Head Segments
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