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Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
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Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

English

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5,726 Views

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08:40 min

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May 02, 2019

DOI:

10.3791/59398-v

DOI:
10.3791/59398-v

•

08:40 min
May 02, 2019

•

1 Views
,
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, 2VA Ann Arbor Healthcare System

Summary

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This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples.

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Cite this Article

Pearson, G., Soleimanpour, S. A. Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay. J. Vis. Exp. (147), e59398, doi:10.3791/59398 (2019).

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