Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Visualization of Endogenous Mitophagy Complexes…
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.

JoVE Journal Medicine
Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft
Journal (Medicine)
A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft
Development and Implementation of a Multi-Disciplinary Technology Enhanced Care Pathway for Youth and Adults with Concussion
Journal (Medicine)
Development and Implementation of a Multi-Disciplinary Technology Enhanced Care Pathway for Youth and Adults with Concussion
 

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Article DOI: 10.3791/59398-v • 08:40 min • May 2nd, 2019
May 2nd, 2019 •
1, 1,2
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, 2VA Ann Arbor Healthcare System

Chapters

Summary
Automatic Translation

This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples.

Tags

Endogenous Mitophagy Complexes Visualization Proximity Ligation Assay Human Pancreatic Beta Cells Mitophagy Research Translationally-relevant Tissues Rare Availability Small Sample Numbers Isolation Protocol Culture Human Islet Equivalents Complete Pancreatic Islet Medium Dissection Light Microscope Treatment Or Condition Of Interest 1.5 Milliliter Tubes Sedimentation Centrifugation Resuspension Pellet PBS Supplemented With PR619 Deubiquitinase Inhibitor Dissociation Of Islets Into Single Cells Trypsin Inhibitor Mixture
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter