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Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells
Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells
JoVE Journal
Biology
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JoVE Journal Biology
Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells

Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells

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07:04 min

September 15, 2023

DOI:

07:04 min
September 15, 2023

1136 Views
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Summary

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This protocol outlines two methods for the quantitative analysis of mitophagy in pancreatic β-cells: first, a combination of cell-permeable mitochondria-specific dyes, and second, a genetically encoded mitophagy reporter. These two techniques are complementary and can be deployed based on specific needs, allowing for flexibility and precision in quantitatively addressing mitochondrial quality control.

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