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September 15, 2023
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This protocol outlines two methods for the quantitative analysis of mitophagy in pancreatic β-cells: first, a combination of cell-permeable mitochondria-specific dyes, and second, a genetically encoded mitophagy reporter. These two techniques are complementary and can be deployed based on specific needs, allowing for flexibility and precision in quantitatively addressing mitochondrial quality control.
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Cite this Article
Levi-D’Ancona, E., Sidarala, V., Soleimanpour, S. A. Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells. J. Vis. Exp. (199), e65789, doi:10.3791/65789 (2023).
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