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Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligati...

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Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

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09:34 min

December 10, 2016

DOI: 10.3791/54899-v

Emily Tubbs¹, Jennifer Rieusset²

¹Lund University Diabetes Centre,Lund University, ²INSERM UMR-1060, CarMeN Laboratory, Lyon 1 University, INRA U1235, INSA of Lyon,Rockefeller and Charles Merieux Lyon-Sud Medical Universities

Here, we describe a procedure to visualize and quantify with high sensitivity the endogenous interactions between the endoplasmic reticulum and mitochondria in fixed cells. The protocol features an optimized in situ proximity ligation assay targeting the inositol 1,4,5-triphosphate receptor/glucose-regulated protein 75/voltage-dependent anion channel/cyclophilin D complex at the mitochondria-associated membrane interface.

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