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Chapters
- 00:04Title
- 00:47Preparation of the Peristaltic Liquid Handler Programs
- 01:53Experimental Design and Generation of the Picklists to Drive the Acoustic Droplet Ejection (ADE)-Based Dispenses
- 03:34DNA Source Plate Preparation Using the 384-well Pipetting Guide Application and Survey Performance to Control the Manually Dispensed Volumes
- 06:18Peristaltic Liquid Handler-Based 1 μL Diluent Dispensation in the Destination Plate
- 07:03ADE-driven DNA Dispensing into the Destination Plate and ADE-driven Transfection Reagent Dispensing
- 09:54Peristaltic Liquid Handler-Based Cell Dispensation
- 10:51Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections
- 12:26Conclusion
This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.
