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Genetics

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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
 

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Article doi: 10.3791/59570
August 8th, 2019

Summary August 8th, 2019

This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.

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