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Cancer Research

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JoVE Journal Cancer Research
Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Article DOI: 10.3791/59633-v • 09:42 min • June 26th, 2019
June 26th, 2019 •
1,2,3, 1,2,4, 1,2, 1,2, 1,2,4, 1,2,3,4, 1,2,3,5, 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Integrated Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center

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Summary
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We describe two methods for assessing transient vascular permeability associated with tumor microenvironment of metastasis (TMEM) doorway function and cancer cell intravasation using intravenous injection of high-molecular weight (155 kDa) dextran in mice. The methods include intravital imaging in live animals and fixed tissue analysis using immunofluorescence.

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Tumor Microenvironment Metastasis Vascular Permeability Intravital Imaging Fixed Tissue Analysis Dextran Tumor Vasculature Multiphoton Imaging Equipment TMEM Cancer Cell Dissemination Metastatic Potential Transplantation DMEM/F12 Cell Culture Medium
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