This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution.
Harney, A. S., Wang, Y., Condeelis, J. S., Entenberg, D. Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment. J. Vis. Exp. (112), e54042, doi:10.3791/54042 (2016).