Journal
/
Medicine
/
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

DOI:
10.3791/54042-v

•

08:24 min

•

June 12, 2016

•

, , ,
1Department of Anatomy & Structural Biology,Albert Einstein College of Medicine, 2Department of Radiology,Albert Einstein College of Medicine, 3Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, 4Integrated Imaging Program,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 00:40Imaging Preparation
  • 02:19Indwelling Tail Vein Catheter Insertion and Mammary Tumor Exposure
  • 03:41Image Acquisition
  • 06:03Results: Visualizing Vascular Permeability
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution.

Tags

Keywords: Intravital ImagingTumor MicroenvironmentMultiphoton MicroscopyVascular PermeabilityMulticellular InteractionsKineticsAnesthesiaTail Vein CatheterizationSubcutaneous Incision

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54042?article_type=v

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
check_url/54042?article_type=v

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
check_url/54042?article_type=v

MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression
check_url/54042?article_type=v

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
check_url/54042?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/54042?article_type=v

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
check_url/54042?article_type=v

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
check_url/54042?article_type=v

Chemotherapy-induced Vascular Toxicity – Real-time In vivo Imaging of Vessel Impairment
check_url/54042?article_type=v

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
check_url/54042?article_type=v

Real-time X-ray Imaging of Lung Fluid Volumes in Neonatal Mouse Lung

Read Article