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Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
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Medicine
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JoVE Journal Medicine
Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

DOI:
10.3791/50088-v

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08:26 min

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March 24, 2013

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, ,
1Watson School of Biological Sciences, 2Cold Spring Harbor Laboratory, 3Departments of Medical Genetics,University of Oslo and Oslo University Hospital

Chapters

  • 00:05Title
  • 01:16Pre-treatment of Animals for Imaging and Preparation of Surgical Tools and Platform
  • 02:31Exposing the Inguinal Mammary Gland for Imaging
  • 04:21Positioning the Mouse on the Stage
  • 06:16Representative Live Imaging Results
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for imaging response to anti-cancer treatment in vivo and at single cell resolution.

Tags

Keywords: Live ImagingDrug ResponseTumor MicroenvironmentMouse ModelsBreast Cancer3D Co-cultureTumor-stroma InteractionsSpinning Disk Confocal MicroscopySystemic TherapyCell LabelingSurgical ProcedureTime-lapse MoviesImage Analysis

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