Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
 

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Article DOI: 10.3791/61371
June 9th, 2020

Chapters

Summary June 9th, 2020

We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter