Engineering
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基于数字微流体的多功能套件，用于科学教育
Chapters
Summary April 26th, 2021
Please note that all translations are automatically generated.
Click here for the English version.
我们描述了一个教育工具包，允许用户执行多个实验，并获得数字微流体的实践经验。
Transcript
我们提供基于商业来源的印刷电路板的数字微流体教育套件，使用户能够亲身体验数字微流体。只要可以共享数字多氯联苯设计文件，这是一个可行的低成本教育解决方案。我们建议使用数字微流体作为教育工具，因为液滴在通用电极阵列平台上纵。
用户可以利用一组扩展的电子元件，这些电子元件现在非常方便自己使用，以便与液滴进行电子接口。展示程序的将是杨实验室的研究生郭宇浩。首先将表面安装电阻器、晶体管和发光二极管焊接到PCB板上。
将高压电源板的输出与焊接组件连接到多氯联苯板。然后将电池连接到电压助推器板，将电压从 6 伏提高到 12 伏。将湿度传感器、超声波压电雾化器和雾化器驱动板连接到微控制器板。
使用所提供的补充代码打开微控制器。调整高压板的可变电阻，使用数字多米测量 EWOD 电极的电压。戴上干净的氮化物手套，使用微皮在电极区域涂抹 10 微升的 5 分石硅油。
用手指均匀地将油铺在电极区域。切一块尺寸为2.5厘米的食品包装，放在电极顶部。使用微管在电极区域涂抹硅油，均匀分布。
要进行化学发光实验，使用微皮在目标电极上放置两到五微升的发光溶液。将10微升0.1%的铁氰化钾放在电极上，电极可以作为电击液滴移动。打开微控制器，使铁氰化钾液滴与发光醇合并。
对于荧光成像，切一平方厘米的半透明胶带，并将其放置在激发发光二极管和 EWOD 电极之间。用胶带将排放玻璃滤镜连接到智能手机的摄像头上，并将 10 微升的铁氰化钾溶液放在电极上。使用智能手机录制液滴激活的视频。
对于长期液滴激活，将一毫升水放在超声波雾化器上。放置一滴铁氰化钾，然后打开微控制器。然后立即关闭外壳的盖子。
一小时后检查液滴激活。此处显示了具有代表性的液滴运动。在化学发光实验中，铁氰化物液滴在12秒内被激活，与目标电极上预先沉积的发光液滴移动并混合。
此处显示了 LED 作为激发光源的示意图设置、用作光扩散器的半透明透明办公胶带以及直接连接到智能手机摄像头的排放过滤器。在黑暗中含有荧光素异氰酸酯的液滴的荧光成像被视为半透明胶带的结果，作为扩散器，均匀地分配激发光。对于长期实验，可以观察到成功的液滴激活。
此处显示了超声波雾化器作用下的代表性湿度数据。此协议可用于开发基于数字微流体的教育套件。据报道，一个基于发光的化学发光实验是一个具体的例子。
该套件可在短时间内组装，电子产品培训最少。此处描述的简化实验可扩展到其他实验。例如，纸试剂盒可以通过将液滴移动到纸张上进行吸收来使用。
还可添加带接口逻辑电路的微控制器，以提供更复杂的数字控制和可编程性。此协议可使非专业爱好者能够学习和应用电子产品，以进一步提升他们对该领域的知识。
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