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JoVE Journal
Biochemistry

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Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
 

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Article DOI: 10.3791/62180-v
February 4th, 2021

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Summary February 4th, 2021

Presented here is a procedure to express and purify myosin 5a followed by a discussion of its characterization, using both ensemble and single molecule in vitro fluorescence microscopy-based assays, and how these methods can be modified for the characterization of nonmuscle myosin 2b.

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