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JoVE Journal
Developmental Biology

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An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
 

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Article DOI: 10.3791/62693-v 11:02 min August 13th, 2021
August 13th, 2021

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Summary

Detailed step-by-step protocols are described here for studying mechanical signals in vitro using multipotent O9-1 neural crest cells and polyacrylamide hydrogels of varying stiffness.

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Tags

Optimized O9-1/Hydrogel System Mechanical Signals Neural Crest Cells Chemical Signals Molecular Mechanisms Genetic Mechanisms Neural Crest Development Diseases Glass Cover Slips Transfer Process Sterilization Sodium Hydroxide Aminopropyl Triethoxysilane (APTS) Rinse Process
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