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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

DOI:
10.3791/60051-v

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09:33 min

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October 03, 2019

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, , , , , , ,
1Centre for Craniofacial and Regenerative Biology,King’s College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research,University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics,King’s College London, 4Department of Biological Applications and Technology,University of Ioannina

Chapters

  • 00:04Title
  • 01:05Early Somite Stage Embryo Dissection (Day 1)
  • 03:12Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
  • 04:36Neural Crest Single Cell Tracking
  • 05:22Quantification of Neural Crest Cell Migratory Capacity
  • 06:04Quantification of Neural Crest Cell Area and Circularity
  • 07:00Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape changes.

Tags

Keywords: Primary Cranial Neural Crest CellsNeural Crest Cell DelaminationNeural Crest Cell MigrationNeural PlateEmbryonic DissectionNeural Crest DevelopmentMouse EmbryoCell CultureDevelopmental Biology

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