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Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
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Bioengineering
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JoVE Journal Bioengineering
Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

DOI:
10.3791/3149-v

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07:26 min

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August 17, 2011

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, , ,
1Department of Integrative Biology and Pharmacology,The University of Texas Health Science Center at Houston

Chapters

  • 00:05Title
  • 01:41Cell Preparation
  • 02:52Calcium Assay Preparation
  • 04:37Plate Reading in FlexStation 3 and Data Analysis
  • 05:48Representative Calcium Assay Results
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

This video provides a detailed protocol for studying the pharmacological profile of human TRPA1 channels using FlexStation 3. The protocol covers details of cell preparation, dye loading and operation of the microplate reader, FlexStation 3.

Tags

Cell-based Calcium AssayMedium To High Throughput ScreeningTRP Channel FunctionsFlexStation 3Multi-mode Microplate ReaderFluorescence MeasurementMulti-well PlatesAcademic SettingsIntegrated Fluid Transfer ModuleMulti-channel PipetterFluorescence ChangesCa2+-permeable Ion ChannelsG-protein Coupled ReceptorsIntracellular Free Ca2+ LevelsTransient Receptor Potential (TRP) ChannelsCalcium InfluxTRPA1 ChannelMolecular SensorNoxious StimuliHEK293 CellsCa2+-sensitive Fluorescent DyeFluo-4Real-time Fluorescence ChangesTRPA1 AgonistTRPA1 Antagonist

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