A set of protocols are presented that describe the measurement of contractile function via sarcomere length detection along with calcium (Ca2+) transient measurement in isolated rat myocytes. The application of this approach for studies in animal models of heart failure is also included.
Lavey, E. A., Westfall, M. V. Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes. J. Vis. Exp. (183), e64055, doi:10.3791/64055 (2022).