Journal
/
Medicine
/
Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

DOI:
10.3791/64055-v

•

07:32 min

•

May 25, 2022

•

,
1Department of Cardiac Surgery, Michigan Medicine,University of Michigan, Ann Arbor

Chapters

  • 00:05Introduction
  • 00:40Contractile Function Analysis of Adult Rat Cardiac Myocytes
  • 04:20Data Analysis of Contractile Function in Isolated Myocytes
  • 06:18Results: Contractile Function in Cardiac Myocytes in Response to Pressure Overload (PO) and Gene Transfer
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

A set of protocols are presented that describe the measurement of contractile function via sarcomere length detection along with calcium (Ca2+) transient measurement in isolated rat myocytes. The application of this approach for studies in animal models of heart failure is also included.

Tags

Keywords: Cardiac Contractile DysfunctionCa2+ TransientsRodent MyocytesHeart FailureContractile FunctionNeurohormonesTherapeutic AgentsSarcomere Length ImagingContractile Function PlatformPeristaltic PumpCell StimulatorMedia PerfusionCover Slip ChamberMicroscopeHeating SystemPacing Stimulator

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64055?article_type=v

Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats
check_url/64055?article_type=v

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo
check_url/64055?article_type=v

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
check_url/64055?article_type=v

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
check_url/64055?article_type=v

Isolation of Atrial Myocytes from Adult Mice
check_url/64055?article_type=v

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
check_url/64055?article_type=v

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
check_url/64055?article_type=v

Fertility Preservation in Patients with Severe Ovarian Dysfunction
check_url/64055?article_type=v

An Intact Pericardium Ischemic Rodent Model
check_url/64055?article_type=v

A Rodent Model of The Ross Operation: Syngeneic Pulmonary Artery Graft Implantation in A Systemic Position

Read Article