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Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

DOI:
10.3791/61562-v

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09:20 min

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November 12, 2020

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, , , ,
1Center for Discovery and Innovation,Hackensack Meridian Health Center, 2Department of Chemistry and Chemistry Biology,Stevens Institute of Technology

Chapters

  • 00:04Introduction
  • 00:56Isolation of CB-SC-derived Exosomes
  • 02:03Isolation of Human CD14-positive Monocytes
  • 04:07Treatment of Monocytes with CB-SC-derived Exosomes
  • 06:08Characterization, Interaction and Effects of the CB-SC-derived Exosomes on the Human Peripheral Blood Mononuclear Cells
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages.

Tags

Keywords: Monocyte DifferentiationMacrophageCord Blood Stem CellExosomesImmune ModulationCD14+ CellsCell SeparationUltracentrifugationStem Cell Educator Therapy

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