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Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
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Cancer Research
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JoVE Journal Cancer Research
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

DOI:
10.3791/56311-v

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06:00 min

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September 13, 2017

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, , , , , , , ,
1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST),IRCCS

Chapters

  • 00:05Title
  • 01:17Osteoclast Differentiation and Cancer Cell Co-culture
  • 04:06Results: Representative Effects of Everolimus on Osteoclastogenesis Treatment
  • 04:51Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes development of an in vitro human preclinical model of osteoclastogenesis from peripheral blood monocytes cultured with breast cancer cell lines to mimic the cancer cell-osteoclast interaction. The model could be used to further our understanding of bone metastasis formation and improve therapeutic options.

Tags

Keywords: OsteoclastogenesisPeripheral Blood MonocytesBreast Cancer Cell LinesBone MetastasisPreclinical ModelPBMCMCSFTRAP Staining

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