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Journal
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Biology
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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
単一核の配列のための凍結した哺乳類ティッシュからの単一核の隔離のための簡単で、速く、部分的に自動化されたプロトコル
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
単一核の配列のための凍結した哺乳類ティッシュからの単一核の隔離のための簡単で、速く、部分的に自動化されたプロトコル
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/65611-v
•
01:58 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Keywords: Single Nuclei Isolation
Single Nucleus Sequencing
Automated Nuclei Extraction
Standardized Protocols
Drug Discovery
Transcriptomics
Gene Expression
Robotic Dissociation
Chemical Gradient Filtration
Automated Cell Counting
Mouse Brain
Rat Kidney
Cynomolgus Liver And Spleen
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単一核の配列のための凍結した哺乳類ティッシュからの単一核の隔離のための簡単で、速く、部分的に自動化されたプロトコル
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