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Journal
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Biology
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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
Um Protocolo Simples, Rápido e Parcialmente Automatizado para o Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de Núcleo Único
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Um Protocolo Simples, Rápido e Parcialmente Automatizado para o Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de Núcleo Único
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/65611-v
•
01:58 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Keywords: Single Nuclei Isolation
Single Nucleus Sequencing
Automated Nuclei Extraction
Standardized Protocols
Drug Discovery
Transcriptomics
Gene Expression
Robotic Dissociation
Chemical Gradient Filtration
Automated Cell Counting
Mouse Brain
Rat Kidney
Cynomolgus Liver And Spleen
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