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Establishing In Vitro LLEC Cultures Using Magnetic Cell Selection

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Unveiling Cellular Functions and Potential Clinical Implications of Leptomeningeal Lymphatic Endothelial Cells (Video) | JoVE

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Harvest and Primary Culture of Leptomeningeal Lymphatic Endothelial Cells

Unveiling Cellular Functions and Potential Clinical Implications of Leptomeningeal Lymphatic Endothelial Cells (Video) | JoVE

Establishing In Vitro LLEC Cultures Using Magnetic Cell Selection

DOI:

10.3791/65872-v

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01:08 min

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September 08, 2023

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Hong-Ji Deng, Kun Wu, Han-Fu Yu, Yong-Jin Zhang³, Yun-Cong Li, Chong Li, Fei Wang

¹Department of Neurosurgery,The First Affiliated Hospital of Kunming Medical University, ²Department of Clinical Laboratory,The First Affiliated Hospital of Kunming Medical University, ³Clinical Medical Research Center,The First Affiliated Hospital of Kunming Medical University

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Keywords: Leptomeningeal Lymphatic Endothelial Cells LLEC Primary Culture Harvest Isolation VEGF-C LYVE-1 MACS Immunofluorescence Flow Cytometry

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