Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Anterior High-Resolution Optical Coherence Tomography, or HROCT, is a promising tool or non-invasively characterizing Ocular Surface Squamous Neoplasia, or OSSN. It provides an In Vivo cross sectional volume of ocular surface lesions, offering an optical biopsy. For OSSN, we presented a standardized practical, reproducible approach to assist clinicians in managing OSSN in clinical workflows.

HROCT can expanded play assist in guiding the differentiation of OSSN from various ocular lesions. HROCT images of OSSN displayed three distinct features:hyper reflective epithelial sickening, a rapid transition from normal epithelium to abnormal tissue, and a distinct claim between the lesion and underlying tissues. Most non epithelial derived diseases usually present as normal epithelium with thickening sub epithelial visions.

The imaging biomarkers in ASOCT of OSSN can be extended further in deep learning applications. This can help ophthalmologists increase their diagnostic, prognostic, and monitoring accuracy, as well as decrease workloads and costs. To begin the examination, ask the subject to sit behind the slit lamp and explain the testing procedures to the subject.

Edit the subject's information. Disinfect the setup by wiping down the slit, lamp head, and chin rest with an alcohol swab. To position the subject for examination, place their chin on the chin rest.

Ask the subject to rotate the eyeball to fully expose the lesion and observe the lesion under the slit lamp. Then perform anterior segment photography with the slit lamp to document clinical features. Flip the eyelids to observe whether fornical or palpebral conjunctiva are affected.

Note all the listed details of the lesion during the clinical examination. Ask the subject to sit behind the imaging device and explain the testing procedures to the subject. Then double click on the RTV icon to start the procedure.

Click on the new patient button to create a new patient and fill out the information fields such as last name, first name, gender, birthdate, and ethnicity. Click on the save button. Now disinfect the setup by wiping down the OCT head and chin rest with an alcohol swab.

Select the desired scan pattern by clicking on the cross line, followed by the cornea button. Attach the anterior segment lens to the system scanner, then select the eye to be scanned according to the anterior segment slit lamp photography. Ask the subject to place their chin on the chin rest and instruct the subject to roll the eyeball to fully expose the ocular surface lesion.

Align the lesion of interest with the center of the scan. Click on the scan tab. Afterward, press auto P to enhance signal strength.

Adjust the scanner head to get the best ocular image. Capture the image using the eight millimeters by eight millimeters automated segmentation OCT scan. Review the OCT slices before saving the scan.

To determine the region of interest, observe the dynamic sectional view during alignment. Align the cross line with the thickest point of the lesion and capture the scan. Pay attention to the adjacent parts of normal and abnormal tissue.

Capture the border of the lesion. Open the system software and locate the existing patient by typing their name or birth date in the search by space. Input the patient's relevant information and click on the search button.

Once the patient is located, click on their name to list all the visits. Choose the latest visit and review the OCT images. Click on the scan button to obtain the current ASOCT image as demonstrated previously and assess the parameters.

To begin the optical coherence tomography or OCT image analysis, open the system software. Navigate to the measuring tool section and click on the view B scans icon. Then within the measuring tools, select the distance tool to measure the maximal epithelial and sub epithelial thickness values of the lesion.

Do so by choosing an initial point on the anterior epithelium boundary. Then move vertically to the visible separation between the epithelial and sub epithelial layers. Save the maximal area measurement data as epithelial thickness.

Next, select an anchor point on the visible separation between the epithelial and sub epithelial layers, and proceed vertically to the posterior border of the lesion, which is typically the anterior boundary of the sclera. Save the maximal area measurement data as subepithelial thickness. Utilizing the snapshot tool icon, export a JPG image of the measurement report screen.