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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Bioengineering
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JoVE Journal Bioengineering
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

DOI:
10.3791/57814-v

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12:22 min

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August 04, 2018

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, ,
1Department of Medicine,Boston University School of Medicine, 2Department of Biomedical Engineering,Boston University

Chapters

  • 00:04Title
  • 01:15Oblique Scanning Laser Ophthalmoscopy (oSLO) System Setup
  • 04:09Optical Coherence Tomography System Setup
  • 05:17Data Acquisition
  • 06:46In Vivo Retinal Imaging
  • 10:07Results: Rat Retinal Image Acquired by Simultaneous oSLO and OCTA
  • 11:35Conclusion

Summary

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Here, we present a protocol to get a large field of view (FOV) three-dimensional (3D) fluorescence and OCT retinal image by using a novel imaging multimodal platform. We will introduce the system setup, the method of alignment, and the operational protocols. In vivo imaging will be demonstrated, and representative results will be provided.

Tags

Keywords: MultimodalVolumetricRetinal ImagingOblique Scanning Laser Ophthalmoscopy (oSLO)Optical Coherence Tomography (OCT)Blood-retina Barrier DisruptionRetinal Capillary FunctionsDiabetic RetinopathyPre-retinal DiseasesIn Vivo ImagingMouse CortexSuper-continuum LaserDichroic MirrorPrismSpectrometerOptical Fiber CouplerGalvanometer MirrorTelescope SystemOblique IlluminationTomographic Fluorescence Imaging

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