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Immunology and Infection
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Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

DOI:
10.3791/57104-v

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07:51 min

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October 21, 2022

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, , , , , , ,
1Vision Institute,Sorbonne University, UM 80 / INSERM, UMR_S 968 / CNRS, UMR_7210, 2Quinze Vingts National Ophthalmology Hospital, 3Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute,Johns Hopkins University School of Medicine, 4Laboratory for Optics and Biosciences (LOB),École polytechnique, 5LOA – ENSTA Paris,École polytechnique

Chapters

  • 00:04Introduction
  • 00:47Sample Preparation
  • 01:31FF‐OCM Initialization, Setup, and Image Acquisition
  • 03:48Image Analysis: Qualitative and Quantitative Assessment of Stromal Morphology and Features
  • 06:08Results: Qualitative and Quantitative Assessment of Corneal Donor Stroma
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

We describe use of full-field optical coherence microscopy as a method for high quality assessment of corneal donor stroma. This protocol can be used to identify features indicative of health or disease, and is aimed at improving the screening and selection of donor tissues, and hence the results of keratoplasty.

Tags

Keywords: CorneaCorneal GraftOptical Coherence MicroscopyHistologyStromal FeaturesEye BanksCornea TransplantationHigh ResolutionImmersion MediumSample HolderCover SlipGreen LEDAcquisition SoftwareMacro ImageAuto-adjustmentJoystickDepth AdjustmentAveragingCorneal Surface

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