Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Our research investigated monocyte and macrophage lineage, emphasizing its remarkable plasticity and ability to integrate multiple signal from the environment to shape the effector response during inflammation, tissue repair, and infections. Macrophage are found throughout the body and coordinate the initiation and resolution of the innate and adapted immunity impacting the protected and immune-mediated pathology. Reprogramming macrophage is the most promising feature in this field.

Emerging omics technology have revolutionaried our understanding of macrophage biology, providing insight into their phenotypic diversity, the functional characteristic, their programming potential, and the developmental origin of these cells. The main obstacle and pitfall in discovering macrophage differentiation and polarization is the heterogeneous experimental conditions and protocols across the literature and the lack of consensus in defining the macrophage term. We demonstrate the reprogramming effect of drug and lipid mediator on macrophage polarization and develop a 3D in vitro model of interaction between macrophage and glioblastoma cells.