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Immunology and Infection
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In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

DOI:
10.3791/60541-v

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08:08 min

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November 01, 2019

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, , ,
1Heart Research Institute, 2Sydney Medical School,University of Sydney, 3Department of Biomedical Sciences,University of Copenhagen

Chapters

  • 00:05Title
  • 01:35Polarization of HMDM (Human Monocyte-derived Macrophages)
  • 02:36Stimulation of HMDM to Induce MET (Macrophage Extracellular Traps) Release
  • 04:20Visualization of MET in Live Cell Culture
  • 05:46Results: Morphological Changes of Differentially Polarized HMDMs and Release of METs Following Stimulation
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

Tags

Keywords: Extracellular TrapMacrophagesNeutrophilsImmune CellsInnate ImmunityInflammatory PathologiesIn Vitro ModelPrimary Human CellsMonocyte-derived MacrophagesM1 PrimingM2 PrimingHypochlorous AcidPMATNF-alphaIL-8 Stimulation

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